Figure 8.

Experimental design of clinically relevant ovine grafted burn wound healing model. The third-degree burn was induced by fire flame (above left). 24 h after the burn, the eschar was excised down to the fascia. Split-thickness skin grafts 0.03 inches thick were harvested from sites remote from the burned sites to be meshed and grafted to the wounds (above center). 0.1 ml/cm² of 4% Omega-7 in 0.9% saline was topically applied to one of two randomly selected autografted sites (Omega-7 group). The remaining site received the same amount of 0.9% saline as a control (control group). The donor sites were also randomly allocated to 0.1 ml/cm² of 4% Omega-7 or 0.9% saline (above right). After the initial treatment, the grafted sites were covered with tie-over dressings removed at postoperative day (POD) 7 (below left). At the 7 days after the grafting, the wounds were gently washed daily with a sterile saline without anesthesia (below center). Thereafter, the wounds were treated with Omega-7 or 0.9% saline, and covered with polyurethane foam for 7 days (14 days total duration of experiments). The donor site wounds were covered with polyurethane film dressing and treated daily with 4.0% Omega-7 or 0.9% saline starting at POD1 for 14 days (below right). The study design was drawn using Adobe illustrator by the first author (https://www.adobe.com/jp/products/illustrator.html).