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. 2021 Jan 13;11:853. doi: 10.1038/s41598-020-79578-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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The depletion protocol of CD206+ macrophages (MΦ) in the CD206 DTR mouse-derived endometriosis model. After one week of inoculation of endometrial fragments derived from donor mice (①), the formation of an endometriotic-like lesion in the peritoneal cavity was confirmed in some mice. (②). The recipient mice underwent intraperitoneal injection of Diphtheria Toxin (DT) for DT group or phosphate buffered salts (PBS) for control group every two days starting from day 7 to day14 after endometrial inoculation. After 2 weeks of inoculation of the endometrium, the total weights of endometriotic-like lesions per mouse were investigated. Estrogen was injected every week.