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. 2021 Jan 13;11:981. doi: 10.1038/s41598-020-80035-4

Figure 2.

Figure 2

Lipid droplets (LD) number evaluation in the normal immortalized esophageal cell line, Het1a, upon exposure to esophageal tumors risk factors associated stimuli. LD quantification assessed by Oil Red O (ORO) staining following Het1a incubation with 1 µM, 2 µM or 5 µM of Oleic Acid (OA) for 24 h (A); Het1a stimulation with increasing number of acidified medium (pH5.0) (B), or 200 µM Deoxycholic Acid (DCA) (C) 3 min pulses, or the combination of both (D); Het1a treated with increasing concentrations of NNK (Nicotine-derived nitrosamine ketone) (E) and ethanol (F) for 24 h. Experiments were performed in triplicate *p < 0.05; **p < 0.005; ***p < 0.0005.