Skip to main content
. 2021 Jan 13;12(1):81. doi: 10.1038/s41419-020-03369-x

Fig. 1. Ferric iron deposition in the substantia nigra.

Fig. 1

Chemical staining of iron ions showed a gradual increase in iron deposition, 1 (B), 4 (C), and 17 (D) months after the treatment with α-syn PFFs, compared to the control (A) in the ipsilateral side. E–H Chemical staining of iron ions in the control (E) and 1 (F), 4 (G), and 17 months (H) after treatment with α-syn PFFs in the contralateral side. The inset images depict the enlarged small boxes in A–H. I–K Quantitative analyses of the iron deposition density in the substantia nigra (ipsilateral side: I; contralateral side: J; both sides: K). Iron deposition staining levels were quantified with Image-Pro Plus software as the relative optical density above the background. α-Syn, α-synuclein. The data are presented as the mean ± SEM of three independent experiments. n = 3 in each group and time points. *p < 0.05 and **p < 0.01 vs. control group, ##p < 0.01 vs. 1-month group, ΔΔp < 0.01 vs. 4-month group, one-way ANOVA (I, J). *p < 0.05 vs. contralateral group, Tukey’s multiple comparisons test after two-way ANOVA (K). Scale bars: 50 μm in A–H and 50 µm in the insets.