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. 2021 Jan 13;12(1):81. doi: 10.1038/s41419-020-03369-x

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — A–C Double-labeling of chemically stained iron ions (dark brown) along with immunohistochemically stained with TH (A), GFAP (B), or Olig2 (C) antibody (magenta) showed heavy iron deposition 17 months after treatment with α-syn PFFs. The inset images depict the enlarged small boxes in A–C. Scale bars: 200 μm in A and 50 µm in the inset; 50 µm in B, C, and 25 µm in the enlarged images.