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. 2021 Jan 13;12(1):81. doi: 10.1038/s41419-020-03369-x

Fig. 6. Cellular localization of iron deposits in microglia in the globus pallidus.

Fig. 6

Double-labeling of chemically stained iron ions (dark brown) along with immunohistochemical staining using an Iba1 antibody (magenta) shows overlapping of iron deposition in Iba1-positive microglia in the control (A) and 1, 4, and 17 months after the treatments with α-syn PFFs in the ipsilateral side (B–D), control group (E) and 1, 4, and 17 months after treatment with α-syn PFFs in the contralateral side (F–H). I–K Quantitative analyses of iron deposition in microglia in the substantia nigra (ipsilateral side: I; contralateral side: J; both sides: K). α-Syn, α-synuclein. The data are presented as the mean ± SEM of three independent experiments. *p < 0.05 and **p < 0.01 vs. control group, ##p < 0.01 vs. 1-month group, ΔΔp < 0.01 vs. 4-month group, one-way ANOVA (I, J). Scale bars: 50 μm in A–H and 25 µm in the insets.