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. 2021 Jan 13;11:1086. doi: 10.1038/s41598-020-79927-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Pro-atherogenic remodelling and HSP60 expression in thoracic aorta of HFHF diet fed mice. (a) Histological analysis of thoracic aortas stained with H × E (100× and 400×) were subjected to quantification of (b) Intima-media thickness (IMT) (n = 3; Scale bar = 100 µm) and (c) lumen area (n = 3). The sections were also subjected to (d) elastin autofluorescence analysis (upper lane, red arrows indicate elastin breaks; Scale bar = 100 µm) and collagen staining by picrosirius red (lower lane; Scale bar = 50 µm). The graphs represents (e) elastin fragmentation (n = 3), (f) collagen content (n = 5 for control, n = 4 for HFHF) and (g) collagen-to-elastin ratio (n = 4). The expression of HSP60 was analysed by (h) western blotting followed by (i) densitometry (n = 3) and (j) immunohistochemistry (Scale bar = 50 µm; arrows indicate HSP60⁺ stained areas) followed by (k) quantification (n = 3) by Image J. Data were expressed as Mean ± SEM. *p < 0.05, ***p < 0.001 vs Chow diet fed mice, unpaired two-tailed Student’s t-test. L lumen.