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. 2020 Dec 10;23:512–526. doi: 10.1016/j.omtn.2020.12.003

Figure 1.

Figure 1

Translated lncRNAs are hidden in myogenesis

(A) Overview of experimental strategy for transcriptome (RNA-seq) and peptidome. The upper panel shows microscopic images of CPMs during proliferation (CPMs cultured in growth medium [GM]) and the third day of differentiation (CPMs cultured in differentiation medium [DM]). The lower panel is the discovery pipeline for translated lncRNAs. (B and C) Heatmaps of differentially expressed mRNAs (B) and lncRNAs (C) during myoblast proliferation and differentiation, with rows representing mRNAs or lncRNAs and columns representing different myoblast stages. (D) Mass spectrometry results of small peptide encoded by three translated lncRNAs (LNC_003344, LNC_004236, and LNC_004915) as examples. (E) Amino acid length distribution of lncRNA translated peptides detected by mass spectra. (F) Incidence of lncRNA translated peptides detected by mass spectra in each category within RefSeq mRNAs. (G) uORF regulatory network of translated lncRNAs and their potential target genes. lncRNAs containing peptides identified by mass spectrometry were used for the regulatory network analysis.