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. 2020 Dec 26;24(1):101989. doi: 10.1016/j.isci.2020.101989

Figure 1.

Figure 1

Profiling the dynamics of transcriptional responses underlying early osteoclast development in response to RANKL

(A) Schematic representation of the study outline. RNA-seq analysis performed at 0, 4, 14, and 24 hr after RANKL induction in RAW 264.7 cells. Gene clusters (C1-C12), comprising four main modes of transcriptional response: early repressed; late repressed; early activated; late activated.

(B–D) Heatmap (B) and line graphs (C) illustrate for the transcriptional patterns observed in the 12 clusters; the expression patterns of genes with prominent role in osteoclastogenesis are also highlighted here. Overrepresentation analysis (D) using EnrichR online tool depicts the involvement of key pathways in the four modes of responses.