Characterization of exosomes isolated by ExoQuick-TC from mice with or without cecum ligation and perforation (CLP) by (A) western blotting for the exosomal surface markers CD9, TSG101, flotillin-1, CD81, and negative control marker Calnexin using serum samples as a control; (B) the morphology of exosomes as detected by transmission electron microscopy, red arrowheads indicate exosomes; and (C) the average size of the exosomes as quantified by dynamic light scattering analysis. (D) The average size of the exosomes quantified by dynamic light scattering analysis of those affinity-purified subpopulations captured with the Rab5b, CD9, CD31, and CD44 Exo-Flow Exosome Capture Kits.