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Isolated exosomes were further purified using the immune-affinity Exo-Flow Exosome Capture kit, detected by flow cytometry, and plot as forward-scattered light (FSC) versus FITC intensity in the bead flow separation for the various capture antibodies (Rab5b, CD9, CD31, and CD44) coupled with FITC staining. Beads without the biotinylated capture antibodies were used as negative controls.
