Fig 2.

Huaier extract induced G0/G1 phase accumulation of cell cycle in neuroblastoma cells. A. The effect of Huaier extract on cell cycle phase distribution in 3 neuroblastoma cell lines treated with/without Huaier extract (0, 1, 2 mg/ml) in RPMI1640 + 10 % FBS for 24 h followed by analysis of cell cycle, as introduced in methods and materials. Cells were stained with PI for 30 min followed by FACS-can flow cytometer. B. 3 neuroblastoma cells were incubated in RPMI1640 + 10 % FBS with/without Huaier extract (0, 0.5, 1 mg/ml) for 24h. CyclinD3 were detected by western blotting, so was Actin. C. The intensity of CyclinD3 was quantified by ImageJ. After normalization to actin, the relative expression of CyclinD3 versus control group was compared. Data represent mean ± SD. * represents P<0.05.