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. 2021 Jan 1;18(4):1015–1023. doi: 10.7150/ijms.48219

Fig 3.

Fig 3

Effect of Huaier extract on apoptosis and autophagy in neuroblastoma cells. A. 3 neuroblastoma cell lines were incubated in RPMI1640+10% FBS with/without Huaier extract (0, 0.5, 1 mg/ml) for 48h. Expression of cleaved PARP and cleaved-caspase3 were detected by western blotting as introduced in methods and materials, so was Actin. B. The intensity of cleaved PARP and cleaved-caspase3 were quantified by ImageJ. After normalization to actin, the relative expression of cleaved-PARP and cleaved-caspase3 versus control group was compared. Data represent mean ± SD. * represents P<0.05. C. Effect of Huaier extract on autophagy. 3 neuroblastoma cell lines were incubated in RPMI1640 + 10% FBS with/without Huaier extract (0, 0.5, 1 mg/ml) for 24h. Expression of LC3II and LC3I were detected by western blotting as introduced in materials and methods, so was Actin.