Fig 4.
Effect of Huaier extract on MEK/ERK signaling pathways. 3 neuroblastoma cell lines were incubated in RPMI1640 + 10% FBS with/without Huaier extract (0, 0.5, 1 mg/ml) for 24h. Total MEK, p-MEK, total ERK and p-ERK were detected by western blotting as introduced in materials and methods, so was Actin.