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Effect of Huaier extract on mTOR signaling pathway. 3 neuroblastoma cell lines were incubated in RPMI1640 + 10% FBS with/without Huaier extract (0, 0.5, 1 mg/ml) for 24h. p-mTOR2481, p-mTOR2448, mTOR, p-AKT 473, AKT, p-4E-BP1, 4E-BP1 were detected by western blotting as introduced in materials and methods, so was Actin.
