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. 2021 Jan 1;18(4):964–974. doi: 10.7150/ijms.49460

Figure 2.

Figure 2

CX3CL1 expression activated in HUVECs by STAT3 signaling pathway. A-D: The effect of treated with IL-6 on CX3CL1 expression in HUVECs was detected by western blot analysis and qRT-PCR. Cells were stimulated by IL-6 at various concentrations (0, 10, 20, 40 60 and 80, 100 ng/mL) for 24 hours or at different times (0, 6, 12, 24, and 48h) at a concentration of 80ng/mL. All experiments were repeated three times in duplicate, and the semi-quantitative analysis was calculated by SPSS statistical soft. (**P< 0.01). E and F: The effect of treated with overexpression STAT3 or knocked down STAT3 on the expression of CX3CL1 was examined by western blot analysis and qRT-PCR in HUVECs. All experiments were repeated three times in duplicate, and the semi-quantitative analysis was calculated by SPSS statistical soft. (**P< 0.01)