Figure 4.

Representative HE and IHC in BALB/c mice tumor-bearing experiment. (A) Histopathological assessment with hematoxylin and eosin staining for tumors from both groups. Representative images of the necrosis of the tumors in each group of mice (200× or 400× magnification); the red arrows indicate tumour-infiltrating lymphocytes (TILs); the green arrows indicate apoptotic cell. (B) Representative images showing CD8 positive staining in the spleen tissues of mice treated with PBS control or the probiotic mixture (200× magnification).