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. 2020 Dec;11(6):1164–1185. doi: 10.21037/jgo-20-474

Table 1. shRNA target sequence of CCAT-1 gene.

Target site Vector Target sequence Transcription product sequence
CCAT-1Homo-111 pGPU6/GFP/Neo-CCAT-1-Homo-111 CCCTTCTTCTAGATTAGCAAT GCCCTTCTTCTAGATTAGCAATTTCAAGAGAATTGCTAATCTAGAAGAAGGGTT
CCAT-1Homo-331 pGPU6/GFP/Neo-CCAT-1-Homo-331 CCTGTACATTGGGAAAGGTGC GCCTGTACATTGGGAAAGGTGCTTCAAGAGAGCACCTTTCCCAATGTACAGGTT
CCAT-1Homo-791 pGPU6/GFP/Neo-CCAT-1-Homo-791 GCACGGATCTGATTTGACTAA GCACGGATCTGATTTGACTAATTCAAGAGATTAGTCAAATCAGATCCGTGCTT
CCAT-1Homo-1068 pGPU6/GFP/Neo-CCAT-1-Homo-1068 CTGGGCCACAAATCAACAACA GCTGGGCCACAAATCAACAACATTCAAGAGATGTTGTTGATTTGTGGCCCAGTT
Empty Vector pGPU6/GFP/Neo-shEV GTTCTCCGAACGTGTCACGT GTTCTCCGAACGTGTCACGTCAAGAGATTACGTGACACGTTCGGAGAATT
Human GAPDH pGPU6/GFP/Neo-shGAPDH GTATGACAACAGCCTCAAG GTATGACAACAGCCTCAAGTTCAAGAGACTTGAGGCTGTTGTCATACTT