Table 1.

Sampling summary for bacterial microbiota analysis

Sample type	Number of samples
E. diaphana (UoM AIMS1–4 cultures): 6 per tank per genotype (6 × 3 × 4)	72
Water (UoM AIMS1–4 cultures): 1 L per tank per genotype (1 × 3 × 4)	12
DNA extraction blanks: tissue ×  1; water ×  1	2
No-template PCR controls: ×  3	3
Artemia salina: ×  3	3
Wild proxy E. diaphana (from an AIMS SeaSim aquarium): ×  5	5
Water (AIMS SeaSim aquarium): ×  1	1
E. diaphana (CC7 16S rRNA gene sequencing data [14])	5
E. diaphana (H2 16S rRNA gene sequencing data [20])	5
Total	108