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. 2019 Nov 14;1:15. doi: 10.1186/s42523-019-0016-0

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Phylogenetic analysis of alcohol dehydrogenase (a) and aldehyde dehydrogenase (b) genes from rumen methanogen genomes and rumen MAGs. Both trees were constructed with the Jones-Taylor Thornton (JTT) model. Saccharomyces cerevisiae ATCC 204508 was used as the outgroup. Numbers represent the relative frequency of branch clustering based on 1000 bootstrap runs, bootstrap values < 50% are removed. Rumen MAGs; MEC1, Organism 3 (Methanosphaera sp.) from the pectin enrichment culture in this study; TAG1265, metagenome assembled Methanosphaera sp. sequences from low methane yield sheep datasets [35]; RUG761, metagenome assembled Methanosphaera sp. sequences from cattle [27]