Figure 3.

Correction effects on coma aberrations. (a) Schematic illustration of the experimental setup. (b) Correction phase patterns at 300, 500, and 800 μm depth. (c) (top) An xy projection image of fluorescent beads observed without applying the correction phase pattern at the depth of 300 μm. (bottom) An xz projection image. (d) (top) An xy projection image of fluorescent beads observed upon applying the correction phase pattern at the depth of 300 μm. (bottom) An xz projection image. (e,f) Bar graphs represent (e) average tilt angles against to the optical axis and (f) average peak intensity of the fitted Gaussian function. The white or black bars indicate average data obtained from fluorescent beads observed with or without applying the correction phase patterns. All error bars represent standard error of the mean (n = 15 beads).