Fig. 6.

Let-7a suppresses mitochondrial OXPHOS in CRC cells. a Barcode enrichment plots of the top two enriched gene sets identified by CAMERA between “let-7a low” and “let-7a high” subgroups in the TCGA CRC cohort based on hallmark gene sets are shown as an example of maximum possible enrichment. Oxygen consumption rate (OCR) was assayed using the Seahorse analyzer (Seahorse Bioscience) in SW620 transfected with let-7a mimic (b) or SW480 transfected with let-7a inhibitor (d) with the treatment of oligomycin, FCCP and a mix of antimycin A and rotenone. c, e Basal OCR and spare respiratory capacity (SRC) were measured in SW620 and SW480 cells. f Western blot analysis of mitochondrial OXPHOS complexes in SW620 and SW480 after the transfection of let-7a mimic or inhibitor. g qPCR analysis of genes mediating OXPHOS in SW620 and SW480 after the transfection of let-7a mimic or inhibitor. Data represent the mean ± SEM of at least three independent experiments. *p < 0.05 and **p < 0.01