Table 1.

Primers used to amplify virulence-associated genes in S. agalactiae strains

Genes	Forward 5′-3′	Reverse 5′-3′	Amplicon (bp)	TM °C
bac	TGTAAAGGACGATAGTGTGAAGAC	CATTTGTGATTCCCTTTTGC	530	50
bca	CAGGAAGGGGAAACAACAGTAC	GTATCCTTTGATCCATCTGGATACG	535	50
rib	CAGGAAGTGCTGTTACGTTAAAC	CGTCCCATTTAGGGTCTTCC	369	50
hylB	TTATCATCCAGCGCCTCCTAG	GTGGTGATAACTGACTTCTTGGGA	245	50
lmb	GACGCAACACACGGCAT	TGATAGAGCACTTCCAAATTTG	300	50
scpB	AGCCATATGCTGCGATCTCT	GGGTTGAACCAAGTGTGCTT	198	58
cylB	GGGCTGCAGGTATTATCGAA	ATTTCCACCAAAAGCAAACG	176	58
fbsA	TGTAGCTAATGGACCGATGTT	TTTTCATTGCGTCTCAAACC	156	58
fbsB	ACAACTGCGGAAATGACCTC	ACGAGCGACGTTGAATTCTT	186	58

bp base pairs