Table 1. Antibodies used in this study.
| Primary Antibody | Secondary antibody (IFA) | Secondary Antibody (WB) |
|---|---|---|
| Rabbit-αAgo2 (EPR10411), Abcam Cat#
ab186733, RRID:AB_2713978 |
Goat-αRabbit, Alexa Fluor 488, Thermo
Fisher Scientific Cat# A-11008, RRID: AB_143165 |
IRDye
® 680RD Donkey anti-Rabbit IgG
Secondary Antibody, LI-COR Biosciences Cat# 926-68073, RRID:AB_10954442 |
| Mouse-αAgo2 (2E12-1C9), Abcam Cat#
ab57113, RRID:AB_2230916 |
Goat-αMouse, Alexa Fluor 488, Thermo
Fisher Scientific Cat# A-11001, RRID: AB_2534069 Goat-αMouse, Alexa Fluor 594, Thermo Fisher Scientific Cat# A-11032, RRID: AB_2534091 |
IRDye
® 680RD Donkey anti-Mouse IgG
Secondary Antibody, LI-COR Biosciences Cat# 926-68072, RRID:AB_10953628 |
| Rat-αAgo2 (11A9), Thermo Fisher Scientific
Cat# 14-6519-82, RRID: AB_2784637 |
Goat-αRat, Alexa Fluor 488, Thermo Fisher
Scientific Cat# A-11006, RRID: AB_2534074 |
- |
| Mouse-α PbHsp70, RRID:AB_2650482 24 | Goat-αMouse, Alexa Fluor 594, Thermo
Fisher Scientific Cat# A-11032, RRID: AB_2534091 |
- |
| Mouse α-αTubulin, Sigma-Aldrich Cat#
T5168, RRID:AB_477579 |
Goat-αMouse, Alexa Fluor 594, Thermo
Fisher Scientific Cat# A-11032, RRID: AB_2534091 |
- |
| Rabbit-α
PfBIP (antibody provided by MR4;
clone MRA19 25, RRID: AB_2716735) |
- | IRDye
® 800CW Donkey anti-Rabbit IgG
Secondary Antibody, LI-COR Biosciences Cat# 926-32213, RRID:AB_621848 |
| Mouse-αHsHsp70, Santa Cruz Biotechnology
Cat# sc-24, RRID: AB_627760 |
- | IRDye
® 800CW Donkey anti-Mouse IgG
Secondary Antibody, LI-COR Biosciences Cat# 926-32212, RRID:AB_621847 |
IFA: Immunofluorescence assay, WB: Western blot.