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. 2020 Dec 22;6(1):762–774. doi: 10.1021/acsomega.0c05340

Figure 4.

Figure 4

Checkerboard titration and flow cytometry analysis against whole cell bacteria. The limit of detection of yPac1A8 for P. acnes DMST 14916 (A) and yPgi3G4 for P. aeruginosa DMST 37186 (C) was determined by checkerboard titration of whole-cell ELISA, using a dilution series of scFv and serial dilutions of bacteria as indicated. The lines represent the average absorbance values of duplicate samples, and error bars represent the standard error of the mean. Pictures of ELISA plates are shown in the Supporting Information (Figures S2 and S3). Flow cytometry analysis of yPac1A8 (B) and yPgi3G4 scFv (D) binding to live P. acnes DMST 14916 and P. aeruginosa DMST 37186, respectively. Reactivity of 6xHis-tagged scFv is indicated by the red line. The secondary antibody alone (control) is indicated by the black line.