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. Author manuscript; available in PMC: 2021 Jan 14.
Published in final edited form as: J Cell Biochem. 2019 Nov 6;121(3):2478–2488. doi: 10.1002/jcb.29470

FIGURE 5.

FIGURE 5

Overexpression of Hdac3 inhibited the odontoblast differentiation of the mDPCs. mDPCs were infected with either control or Hdac3 overexpression plasmid. Then the cells were treated with odontoblastic induction medium. RNA and protein were obtained after 7 day’s induction. qRT-PCR (A) and immunoblots (B) were performed for Hdac3, Dmp1, Dspp, Alp, and normalized to Actin. C, Normalized protein levels were shown in the chart. D, Mineralization was determined by alizarin red staining. All data were based on three independent experiments. mDPCs, mouse dental papilla mesenchymal cells; qRT-PCR, quantitative reverse transcriptase polymerase chain reaction. *P < .05, **P < .01, ***P < .001