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. Author manuscript; available in PMC: 2021 Jan 14.
Published in final edited form as: J Cell Biochem. 2019 Nov 6;121(3):2478–2488. doi: 10.1002/jcb.29470

FIGURE 7.

FIGURE 7

Inhibitors of HDAC3 and p300 could regulate the odontoblast differentiation of the mDPCs. mDPCs were treated with either TSA or C646 for 24 hours, 0.1% DMSO was used as controls, then the cells were treated with odontoblastic induction medium. RNA was obtained after 7 day’s induction. qRT-PCR (A, B) were performed for Dmp1, Dspp, Alp, and normalized to Actin. C, Mineralization was determined by alizarin red staining. D, Normalized mineralization was shown in the chart. All data were based on three independent experiments. mDPCs, mouse dental papilla mesenchymal cells; qRT-PCR, quantitative reverse transcriptase polymerase chain reaction. DMSO, dimethyl sulfoxide; mDPCs, mouse dental papilla mesenchymal cells; qRT-PCR, quantitative reverse transcriptase polymerase chain reaction; TSA, trichostatin A. *P < .05, **P < .01, ***P < .001