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. Author manuscript; available in PMC: 2021 Jan 14.
Published in final edited form as: Nat Cancer. 2020 May 18;1(5):507–517. doi: 10.1038/s43018-020-0061-3

Fig. 2. Reduced suppressive function and altered metabolism of Rel−/− Gr-1+ myeloid cells.

Fig. 2.

a, b, The degree of suppression, at the indicated MDSC:T cell ratio, of CD8+ T cell proliferation by purified splenic Gr-1+ myeloid cells (MDSCs) isolated by FACS from mice that had similar size of B16F10 tumor (a, n=3 mice/group), or generated in vitro from bone marrow of naïve mice (b, n=3 mice/group) (*, P<0.05; **, P<0.01).

c, Mitochondrial oxygen consumption rate (OCR) of bone marrow-derived MDSCs from WT (n=6) or Rel−/− (n=3) mice as measured by Seahorse XF96 analyzer.

d, Basal respiration, maximal respiration, ATP production, and proton leak of WT (n=6 mice) and Rel−/− (n=3 mice) MDSCs measured from the data in c (***, P<0.001).

e, Basal glycolysis and maximal glycolysis of WT (n=6 mice) and Rel−/− (n=3 mice) MDSCs measured using a Seahorse XF96 analyzer (***, P<0.0001). Statistical significance was determined by two-tailed unpaired t-test (a-b, d-e). For all panels, data are presented as means ± s.e.m.