Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jan 5;13(1):1870058. doi: 10.1080/19420862.2020.1870058

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2021 The Author(s). Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 6. — Expression, purification and binding properties of two selected cLC hetero-IgG molecules

A) Final CIEX yields of two cLC hetero-IgGs (A2× B4 and C4×B3) and their corresponding parental mAbs. B) CIEX chromatographs for A2× B4 and C4× B3. C–E) Binding kinetics of two cLC hetero-IgGs (A2×B4 and C4×B3) and respective controls (two hybrid IgGs (HC-A2/LC-B4 and HC-C4/LC-B3) and two parental mAbs (B4 and B3). Representative binding kinetics sensorgrams show processed data overlaid with the global fit to a 1:1 binding model. The weaker binding to antigen-C is rapid equilibrium with a lack of curvature leading to the larger variance in replicate measurements. The binding affinity (K_D) was calculated as mean ± SE from three independent measurements.