Figure 7.
Inhibition of MLCK strongly reduces evoked release by suppressing calcium currents in RBs. A1, Average traces showing that EPSCs recorded in an AII, evoked by puffing LY 341495 (LY), an mGluR6 antagonist, onto the dendrites of RBs located at the OPL, were strongly reduced by application of 100 μm ML-9. Vhold = −80 mV. A2, LY-evoked EPSCs decreased over time with bath application of 100 μm ML-9 (n = 5). The peak amplitudes and integrals of EPSCs were normalized to the amplitude and integral at time 0, respectively, in each cell before averaging across cells. All the data were illustrated as mean ± SEM. B1, Average traces showing that ML-9 (100 μm) strongly suppressed the voltage step-generated calcium currents (ICa) in an RB. B2, Statistics of the effects of 100 μm ML-9 (n = 10) on the peak amplitude and integral of RB ICa. The suppression of ICa recorded in RBs was closely related to the inhibition of eEPSCs recorded in AIIs (adapted from Fig. 6A3, superimposed in gray). All the data were illustrated as mean ± SEM.