Figure 9.

Inhibition of CaM occludes the effect of MLCK inhibition on spontaneous release. A, Representative traces showing that, after preincubation with 50 μm W-7, 100 μm ML-9 did not increase, but instead reduced, AII mEPSC frequency. B, Representative traces showing that, after preincubation with 50 μm W-7, AII mEPSC frequency decreased significantly after 10-min recording. C, Summary data for AII mEPSC frequency under three experimental conditions in A (empty and full circles), B (empty and full squares), and DMSO control (empty and full triangles). The frequencies were normalized to the frequency under control condition in each cell before averaging across cells. The data were also illustrated as mean ± SEM. Wilcoxon signed-rank tests (control 1 vs ML-9, n = 13, p = 0.0002; control 2 vs no drug, n = 8, p = 0.0078; control 3 vs DMSO, n = 7, p = 0.0156) or unpaired Student’s t test (ML-9 vs no drug, p = 0.0001; no drug vs DMSO, p = 0.7337) were used for comparison; *p < 0.05, **p < 0.01, ***p < 0.001; ns: not statistically significant.