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. 2020 Aug 5;162(2):446–458. doi: 10.1097/j.pain.0000000000002033

Figure 7.

Figure 7.

proteinase-activated receptor 2 depletion partially protects male and female mice from developing disulfide HMGB1-induced mechanical hypersensitivity. (A) Representative Western blot images and (B) quantification of proteinase-activated receptor 2 (PAR2) immunopositive bands normalized to glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and presented as percentage change to vehicle control groups. Withdrawal response after i.t. injection of disulfide HMGB1 (1 μg) or vehicle in (C) male and (D) female wild-type (PAR2+/+) and whole-body PAR2 knockout (PAR2−/−) mice. Data are presented as mean ± SEM, n = 6 mice/group for Western blot results and n = 4 to 7 mice/group for behavioral results, *P < 0.05, **P < 0.01, ***P < 0.001 vs control groups. ds HMGB1, disulfide high mobility group box 1 protein.