Figure 5.

Dendrograms showing two examples of immune repertoire mining, using known PTx binders CL-95906 and CL-95940 (leaves colored yellow). Heavy chains predicted to bind PTx are colored as green, blue or purple depending on whether they are predicted to bind via both methods or were clonotype- or paratype-unique. Asterisks indicate heavy chains selected for testing, all of which were validated as PTx binding. A shows sequences predicted to bind PTx that use a different V gene (V459) than the known PTx binder used for prediction (V4-34). Sequences using a different J gene to the known PTx binder are shown in B