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Cytotoxic screening for NF and SFN: A. Human BPH-1 cells were seeded at a density of 1 ×106 cell per milliliter and treated with or without NF (0.625 µM, 1.25 µM, 2.5 µM, 5 µM, 10 µM, 20 µM, and 40 µM) for 24 and 48 h. Cell Counting Kit 8 was used to measure the percentage of cell viability. B. Human BPH-1 cell were seeded at a density of 1 ×106 cells per milliliter and treated with or without SFN (3 µM, 7 µM, 15 µM, 30 µM, and 60 µM) for 24 and 48 h. (n = 5).
