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. 2021 Jan 8;26(1):1–9. doi: 10.1080/13510002.2021.1871814

Figure 2.

Figure 2.

Mechanism of Nrf2 activation by NF: Human BPH-1 cells were treated with NF 3 µM, 9 µM, and SFN 15 µM for 24 and 48 h. A. The gene expression of Nrf2, HO-1, NQO1, Keap1 were normalized with GAPDH B. Protein expression of Nrf2, HO-1, NQO1, Keap1, GAPDH were determined with western blot. C. Relative protein expression of Nrf2, HO-1, NQO1, and Keap1 are normalized with GAPDH. D. Comparison of the Nrf2 and Keap1 relative gene expression at 24 and 48 h and all the values are normalized with GAPDH. E. Comparison of the Nrf2 and Keap1 relative protein expression at 24 and 48 h and all the values are normalized with GAPDH. Values are presented as means ± SD (n = 3). For NF: *P< 0.05, **P< 0.01, ***P< 0.001 compared to control group. For SFN: #P< 0.05, ## P< 0.01, ###P< 0.001 compared to control group (no treatment).