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. Author manuscript; available in PMC: 2021 Jan 14.
Published in final edited form as: FASEB J. 2020 Jul 21;34(9):12024–12039. doi: 10.1096/fj.201902826RR

Figure 2. UBAP2 promotes macropinocytosis by activating KRAS.

Figure 2.

(A) UBAP2 knockdown using siRNA (siUBAP2) decreased the activated RAS (GTP-RAS) but not the total -RAS (Total-RAS) in BxPC3 cells using pulldown-based RAS activation assay. (B) UBAP2 knockdown by siRNA (siUBAP2) decreased KRAS in pancreatic cancer cells expressing constitutively activating KRAS mutation. (C) Activated KRAS rescued the dextran uptake upon UBAP2 silencing (shUBAP2). KRAS G12V (green) and dextran (red) was visualized by IF. Scale bar, 10 μm. (D) Quantification of dextran uptake. Mean ± SD; **, p<0.01; *, p<0.05. Ladder represents molecular weight in KDa.