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. Author manuscript; available in PMC: 2021 Jan 14.
Published in final edited form as: FASEB J. 2020 Jul 21;34(9):12024–12039. doi: 10.1096/fj.201902826RR

Figure 7. UBAP2 Silencing downregulates RAS downstream signaling.

Figure 7.

(A) BxPC3 and (B) PANC1 cells were either transfected with scrambled siRNA (siCon) or siRNA against the UBAP2 gene. 36 h post-transfection cells were starved overnight and then stimulated with EGF (EGF=50ng/ml) for 15 mins at 37°C followed by collection of cell lysate. Cell lysate were than subjected to immunoblot for phospho-ERK, ERK, phospho-p38, P38 phospho-CREB, CREB and Phospho-p70 S6 kinase and p70 S6 kinase. Efficient gene silencing was determined by immunoblotting with UBAP2 antibody. Each experiment was repeated at least 3 times. Ladder represents molecular weight in KDa.