Figure 2.

Generation and characterization of human induced pluripotent stem cells (hiPSCs). (A) Pedigree of the proband with LQTS. Screening of the proband's family revealed prolonged QT in her mother (Patient I-2), her son (Patient III-1), and her elder sister's son (Patient III-3). Squares and circles indicate male and female family members, respectively; solid, open, and line-crossed symbols indicate LQTS diagnosed, unaffected, and deceased family members, respectively. (B) Whole exome sequencing was performed on DNA isolated from peripheral blood samples of the proband and her family. (C) Embryonic stem cell-like clusters. (D) Flow cytometry analysis was performed to determine the percentage of iPSCs expressing the pluripotency surface markers SSEA3 and TRA-1-60. (E1 and E2) Immunofluorescent staining of the pluripotency marker OCT4 in iPSCs. Scale bar.