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. 2021 Jan 14;16(1):e0244996. doi: 10.1371/journal.pone.0244996

Fig 1. Nitrogen-induced expression pattern of OsATG8b gene and confocal analysis of YFP-OsATG8b in rice.

Fig 1

(A) 14-day-old rice seedlings of SN9816 cultured with NS solution transferred to the same NS (2.88 mM N) solution and the ND (0 mM N) solution for 24h (n = 10), the expression of OsATG8b gene in leaf and root. OsActin1 was used as an internal control. Values are means ± SD (n = 3), **P < 0.01 (t-test). (B) Seedlings were grown under NS (2.88 mM N) or ND (0 mM N) conditions with or without 0.5 μM ConA for 14h. Representative confocal microscopy images of the epidermal root cells expressing YFP-OsATG8b in SN9816. The white arrowheads indicate the YFP-OsATG8b labeled autophagosomes. Scale bars, 10 μm.