Table 2. Quality-control information, sequence length-distribution and OTU clustering.
Sample | Raw reads | Clean reads | Base (nt) | Avg. Length (nt) | Q20 | Effective (%) | OTUs | Unique OTUs |
---|---|---|---|---|---|---|---|---|
KK1 | 112427 | 90158 | 38683375 | 429 | 90.59 | 80.19 | 200 | 30 |
KK2 | 95143 | 90373 | 38612344 | 427 | 89.13 | 94.99 | 354 | 116 |
KK3 | 93567 | 90319 | 38764167 | 429 | 87.82 | 96.53 | 204 | 29 |
RE | 78982 | 75132 | 32301400 | 429 | 82.68 | 95.13 | 235 | 81 |
KS | 100129 | 90111 | 38130981 | 423 | 86.47 | 89.99 | 239 | 46 |
Raw reads means reads after filtration; Clean reads means reads after removal of chimeras; Base means base number of nucleotides in clean reads; Avg. Length means average length of clean reads; Q20 means the percentage of bases of quality greater than 20; Effective (%) means the percentage of clean reads relative to raw reads; OTUs means the number of OTUs identified in different samples; Unique OTUs means the number of OTUs only identified in a single sample.