Fig 4. STEC detection and classification by combined qPCR and nanopore sequencing approach.

A) Direct comparison of quantitative qPCR detection with de novo assembly limits by nanopore sequencing informs detection and classification of STECs. B) Pipeline for detection and classification of STECs in enriched irrigation water using nanopore sequencing and EPI2ME cloud-based services to identify reads of a desired taxa for de novo assembly with Flye and in silico analysis.