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. 2021 Jan 14;16(1):e0244439. doi: 10.1371/journal.pone.0244439

Fig 3. SLC15A4 deficient mice are protected in pristane induced lupus model.

Fig 3

Two cohorts of 11-week-old wild-type mice (C57BL/6) and slc15a4-/- mice (on C57BL/6 background) were injected intraperitoneally with either 500μl PBS or pristane. Two weeks post pristane administration, peritoneal lavage was collected from cohort 1, and expression of Interferon signature genes (A-D), secretion of IL12p40 (E) and CCL-2 (F), and number of inflammatory Ly6Chi monocytes (G-H) were assessed by qRT-PCR, Luminex and flow cytometry, respectively. In cohort 2, serum concentrations of anti-nuclear antibodies were assessed 4 months (I) and 9 months (J) after pristane administration. Renal glomerulonephritis was assessed by histological assessment of H&E stained sections after 9 months of pristane administration (K) and scored as described in the methods section (L), and plotted with group means ±SD. *p < 0.05, **p<0.005, ***p<0.0005, N = 7 animals per group (some mice had to be euthanized during study due to fight injuries).