Table 2.
Regression equations, correlation coefficient, linear range, LOD, and recovery of 8 components by HPLC-MS/MS.
| Compounds | Regression equation | r 2 | Linear range (ng/mL) | LOQ (ng/mL) | Recovery (%) |
|---|---|---|---|---|---|
| 1 | Y = 5053.5X − 282.5 | 0.9999 | 0.5-50.0 | 0.5 | 85.4 |
| 8 | Y = 1048721X + 101447 | 0.9999 | 0.5-50.0 | 0.5 | 87.2 |
| 10 | Y = 258201X − 40.1 | 0.9999 | 0.5-50.0 | 0.5 | 83.7 |
| 11 | Y = 508402X − 28108 | 0.9999 | 0.5-50.0 | 0.5 | 88.1 |
| 12 | Y = 1271407X − 111917 | 0.9997 | 2.0-150.0 | 2.0 | 86.8 |
| 13 | Y = 32151X − 3568.5 | 0.9999 | 0.5-50.0 | 0.5 | 86.3 |
| 14 | Y = 211093X − 688.8 | 0.9998 | 0.1-10.0 | 0.1 | 88.2 |
| 15 | Y = 838756X − 77674 | 0.9998 | 0.1-10.0 | 0.1 | 89.5 |
Note: Y is the peak area; X is the concentration injected. LOQ refers to the limit of quantification. 1: chlorogenic acid; 8: jatrorrhizine; 10: coptisine; 11: palmatine; 12: berberine; 13: evodin; 14: evodiamine; 15: rutaecarpine.