Table 2.
S. no. | Strength of the MS medium | Plant growth regulators (mg/L) or additives | Observed in weeks | |
---|---|---|---|---|
Initiation of seed germination | Initiation of protocorm formation | |||
1. | FMS | — | 3 | 5 |
2. | 1/2 MS | — | 3 | 4 |
3. | 1/4 MS | — | 3 | 5 |
4. | FMS | 0.5 BAP + 0.5 NAA | 4 | 7 |
5. | FMS | 1 BAP + 0.5 NAA | 4 | 6 |
6. | FMS | 1.5 BAP + 0.5 NAA | 4 | 6 |
7. | FMS | 2 BAP + 0.5 NAA | 3 | 5 |
8. | FMS | 10% CW | 3 | 4 |
9. | FMS | 5% CW | 3 | 4 |
Culture condition: cultures were maintained under fluorescent light with 8 h dark and 16 h light at 25°C and six replicates were used for each combination.