Table 2.
Characteristics of HPV virus preparations.
| Virus Genotype (stock ID) | Source | Final Virion Isolationa | Physical titre (VGE/mL) | Antibody neutralisation | Dynamic range of infection | VGE per FFUh |
|---|---|---|---|---|---|---|
| HPV11 (10.10) | 293T QV | CsCl density gradient | 1.2 × 1010 | 99% | 3-4 log10 | 3.8 × 104 |
| HPV11 | Xenografts b | Debris Sedimentation at 11,000 x g c | 2.9 × 109 | 98% | 3 log10 | 5.0 × 104 |
| HPV16 (12.35) | 293T QV | CsCl density gradient | 3.6 × 1011 | 99% | 4-5 log10 | 3.8 × 105 |
| HPV16 (12.40) | 293T QV | CsCl density gradient | 1.5 × 1011 | 100% | 4-5 log10 | N.D. |
| HPV16 | HCK 16-8 Rafts | Crude Virus Preparation (CVP) d,e | 1.0 × 109 | 53% | N.D.g | no FFU |
| HPV16 (19.17) | W12-E Rafts | CVP | 2.4 × 107 | 55% | N.D. | no FFU |
| HPV16 (19.40) | W12-E Rafts | CVP | 8.4 × 106 | 40% | N.D. | no FFU |
| HPV31 (18.81) | 293T QV | CsCl density gradient | 3.4 × 1010 | 100% | 3-4 log10 | 1.6 × 104 |
| HPV31 (13.02) | CIN-612 9E Rafts | Virion Sedimentation Ultracentrifugation f | 2.0 × 108 | 97% | ≥5 log10 | 3.0 × 103 |
a
Details of isolation in Methods.
b
Gift from N. Christensen (PennState College of Medicine).
c
VirTis homogenisation, supernatant following debris sedimentation at 11,000 x g.
d
Gift from C. Meyers (PennState College of Medicine).
e
Dounce homogenisation, low speed debris pelleting per Conway et al. 2009.
f
BeadBeater homogenisation, high-speed centrifugation, ultracentrifugation at 130,000 x g; based on sedimentation coefficient of 296S–300S for viral DNA-containing particles.
g
Not determined.
h
Based on RNA-ISH with FFU related to the total VGE exposed to cells.