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. 2020 Oct 29;40(2):277–291. doi: 10.1038/s41388-020-01525-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — A Silver staining of LINC00261-associated proteins. One specific band (arrow) was excised and subjected to mass spectrometry. B Western blot of IGF2BP1 using protein samples enriched by biotinylated LINC00261, anti-LINC00261, and beads from Panc1 and Mia-PaCa2 cells. C Fold enrichment of LINC00261 in RNA samples precipitated by IGF2BP1 or IgG antibody in Panc1 and Mia-PaCa2 cells. The D mRNA and E protein levels of c-myc in Panc1 and Mia-PaCa2 cells with IGF2BP1 knockdown or overexpression. F The protein expression of c-myc in Panc1 and Mia-PaCa2 cells transfected with LINC00261 or cotransfected with LINC00261 and IGF2BP1. G Enrichment of IGF2BP1 measured using the biotinylated UTR region of c-myc in Panc1 and Mia-PaCa2 cells transfected with empty vector or vector with full-length LINC00261. The mRNA stability of c-myc in H Panc1 and I Mia-PaCa2 cells overexpressing LINC00261 or overexpressing LINC00261 and IGF2BP1 after treatment with actinomycin. The data are presented as the mean ± SD of three independent experiments. *P < 0.05. ns no significance.