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. 2021 Jan 14;11:1336. doi: 10.1038/s41598-020-79359-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Anvi’o representation of the pangenome of the 21 Pseudomonas genomes. The first 21 layers represent each genome, and the black coloration signifies the existence of a Gene Cluster (GC). To the right is given an ANI percentage identity heatmap of the same genomes. The subsequent 6 layers correspond to various statistics related to the analysis i.e. the number of contributing genomes per GC, number of genes per GC, maximum number of paralogs per GC, Species Core Genome Clusters, Functional Homogeneity Index and Geometric Homogeneity Index. The last 38 layers represent the various annotation sources for each GC and the green coloration signifies the existence of annotation. “A” represents the common GCs to all 21 strains (Core genome). “B1” represents the GCs unique to the Aeruginosa group strains and “C” represents the GCs which are unique to the Stutzeri group strains. “B2” represents the GCs specific to the Aeruginosa group strains but without Strain05 which has no GCs in this bin. The bar chart at the top right represents the gene redundancy (blue bar color) and the singleton GC (orange bar color) per genome.