Table 1.
Sampling depth, taxonomy assignment and phenotypic traits (i.e. high or low tolerance to acidity, antibiotics and heavy metals) of the 21 strains.
| Strain code | Depth (m) | Closest culture representative | ANI (%) | Main groups | Subgroups |
|---|---|---|---|---|---|
| Low tolerance | |||||
| Strain01 | 45 | Pseudomonas sp. R2A2 | > 97.1 | Stutzeri | Stutzeri 1 |
| Strain02 | 90 | Pseudomonas sp. R2A2 | > 97.1 | Stutzeri | Stutzeri 1 |
| Strain03 | 5 | Pseudomonas sp. R2A2 | > 97.1 | Stutzeri | Stutzeri 1 |
| Strain04 | 495 | P. balearica DSM_6083 | > 98.51 | Stutzeri | Balearica |
| Strain09 | 20 | Pseudomonas sp. MT-1 | > 97.1 | Stutzeri | Xanthomarina 1 |
| Strain10 | 20 | Pseudomonas sp. MT-1 | > 97.1 | Stutzeri | Xanthomarina 1 |
| Strain11 | 20 | P. balearica DSM_6083 | > 98.51 | Stutzeri | Balearica |
| Strain12 | 20 | Pseudomonas sp. R2A2 | > 97.1 | Stutzeri | Stutzeri 1 |
| Strain14 | 90 | P. stutzeri 1W1-1A | > 97.3 | Stutzeri | Stutzeri 2 |
| Strain18 | 90 | Pseudomonas sp. R2A2 | > 97.1 | Stutzeri | Stutzeri 1 |
| Strain22 | 495 | P. balearica DSM_6083 | > 98.51 | Stutzeri | Balearica |
| Strain24 | 495 | P. stutzeri 1W1-1A | > 97.3 | Stutzeri | Stutzeri 2 |
| High tolerance | |||||
| Strain05 | 495 | P. aeruginosa MTB-1 | > 98.97 | Aeruginosa | Aeruginosa |
| Strain06 | 495 | P. aeruginosa MTB-1 | > 98.97 | Aeruginosa | Aeruginosa |
| Strain07 | 495 | P. aeruginosa MTB-1 | > 98.97 | Aeruginosa | Aeruginosa |
| Strain08 | 430 | P. xanthomarina LMG 23572 | 88.8 | Stutzeri | Xanthomarina 2 |
| Strain16 | 495 | P. aeruginosa MTB-1 | > 98.97 | Aeruginosa | Aeruginosa |
| Strain19 | 495 | P. aeruginosa MTB-1 | > 98.97 | Aeruginosa | Aeruginosa |
| Strain20 | 495 | P. aeruginosa MTB-1 | > 98.97 | Aeruginosa | Aeruginosa |
| Strain21 | 495 | P. aeruginosa MTB-1 | > 98.97 | Aeruginosa | Aeruginosa |
| Strain23 | 495 | P. aeruginosa MTB-1 | > 98.97 | Aeruginosa | Aeruginosa |
All strains were closely related to six culture representatives and clustered into six subgroups (i.e. Stutzeri 1 and Stutzeri 2, Balearica, Aeruginosa, Xanthomarina 1 and 2). Strains in bold, representing each of the six subgroups, were used in phylogenetic tree construction presented in Fig. 4.