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. 2021 Jan 14;12:374. doi: 10.1038/s41467-020-20607-0

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a SDS-PAGE gel images of time-dependent formation of LC3B–PE for hAtg3, hAtg3^yNTVar, hAtg3^yNT, hAtg3^yNTCons, hAtg^+7Gly, and for hAtg3^P21A, hAtg3^L23T, hAtg3^E25K as well as hAtg3^V8D_V15K. Gels were quantified with ImageJ; conjugation percentage of LC3B–PE was determined by the amount of LC3B–PE divided by the total LC3B present. CL is the control without liposomes. * Asterisk indicates a small amount of degradation of LC3B in the presence of ATP. b Plots of time-dependent formation of LC3B–PE for hAtg3 and mutants. Data are presented as mean ± SD. Quantification of conjugation reactions were obtained from three separate measurements (n = 3).