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. 2021 Jan 14;14:12. doi: 10.1186/s12284-021-00453-4

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Avr-Pita interacts with OsCOX11. a Analysis of protein–protein interactions between BD:Avr-Pita (mature protease) and AD:OsCOX11 via yeast-two-hybrid (Y2H) assay. Yeast cells harboring the indicated bait and prey plasmids were diluted 10×, 100×, or 1000× and spotted onto selective media SD-LW and SD-AHLW. b In vitro pull-down assays were conducted with fusion proteins (GST:Avr-Pita and His:OsCOX11) using His-Ni beads and detected by immunoblot analysis with anti-GST and anti-His antibodies. Asterisks indicate the target protein. (c) Deletion assays of OsCOX11 to test interactions with Avr-Pita. A series of truncated OsCOX11 proteins were tested for interaction with Avr-Pita via Y2H